Arezoo Tooghi; Gholam Dashab; Ali Maghsoudi; Mohammad Rokouei
Volume 24, Issue 2 , July 2022, , Pages 127-138
Abstract
The aim of this study was to investigate the genetic diversity of different camel breeds in Sistan and Baluchestan province using microsatellite markers. For this purpose, 70 camels were randomly selected from Jamazeh and Baluchi breeds. Jugular vein blood sampling was performed in the tubes containing ...
Read More
The aim of this study was to investigate the genetic diversity of different camel breeds in Sistan and Baluchestan province using microsatellite markers. For this purpose, 70 camels were randomly selected from Jamazeh and Baluchi breeds. Jugular vein blood sampling was performed in the tubes containing 0.5% EDTA. The extraction of DNA was performed by salt-detergent method. Five microsatellite markers were selected from the camel genome project with specific primers and banding patterns on 5% agarose gel. Genetic and population structures were analyzed using POPGENE v1.31 software. The number of alleles in five marker positions in Jamazeh and Baluchi breeds ranged from one to six alleles. The number of alleles in five markers CVRLO6, VOLP32, LCV66, WYLLO and CMS17 in Jamazeh are equal to three, two, three, four and one alleles, respectively, and in Baluchi breed with two, four, two, five and one alleles, respectively. According to the results, the range of heterozygosity observed in the studied sites in Jamazeh and Baluchi breeds was in the range from 0.29 to 0.52. The results of FST analysis showed that the two populations were more than 96% similar and the difference between them was 3%. The results of the presentresearchindicated a low rate of polymorphism in the studied sites and in order to preserve breeds and be more adaptable to environmental conditions, it needs more attention and control of inbreeding.
batol asghari esfedan; Gholam Dashab; mohammadhossein banabazi; mohammad Rokouei
Volume 22, Issue 2 , June 2020, , Pages 187-198
Abstract
The aim of the present study was to investigate the codon usage pattern and their relationship with gene expression for genes with different expression between pure and crossbreed Sistani and Montbeliared breed. In this study, the results of differential gene expression analysis using RNA-Seq technology ...
Read More
The aim of the present study was to investigate the codon usage pattern and their relationship with gene expression for genes with different expression between pure and crossbreed Sistani and Montbeliared breed. In this study, the results of differential gene expression analysis using RNA-Seq technology between pure and crossbreed Sistani and Montbeliared breed (two pure and two its crossbreed) were used. For this purpose, after determining the ORF regions for these genes, CodonW software was used to estimate codon usage pattern indices including CAI, ENC, GC and GC3s. Results showed that there was a significant correlation between total GC and GC3s (0.74). There was also a significant correlation between ENC and GC and GC3s (0.65, 0.77), indicating the role of mutation in codon formation. Based on the results of this study, the factors such as nucleotide composition (GC content), mutation, and gene expression level played important roles in codon formation in the genes studied in this study. This study is the first comparison between pure and crossbreed Sistani and Monti-billiard samples, which helps to better understand the evolutionary mechanisms of codon usage pattern formation and its association with gene expression.
Rokhsareh Najadini; Gholam Dashab; mehdi vafae valleh; nasrollah moradi
Volume 20, Issue 2 , August 2018, , Pages 225-241
Abstract
Heat stress is one of the most important environmental stressors challenging poultry production worldwide, especially in warm regions such as Sistan and Baluchestan province of Iran. Heat stress increases the production of free radicals in the chicken’s body. Glutathione peroxidase plays important ...
Read More
Heat stress is one of the most important environmental stressors challenging poultry production worldwide, especially in warm regions such as Sistan and Baluchestan province of Iran. Heat stress increases the production of free radicals in the chicken’s body. Glutathione peroxidase plays important roles as cellular antioxidants in heat stress. The aim of this study was to conduct an analysis of the evolutionary and phylogenetic of GPX-1 in Ross 308 and Khazak populations. Boold samples were collected from 10 birds selected randomly from two stocks of Khazak and Ross 308 population (5 Ross 308 and 5 Khazak birds). DNA was extracted from whole blood. PCR amplification of 800 bp of GPX-1 was performed using one pairs of special primers. Then, PCR product sent for DNA sequencing. Sequence alignment of the GPX-1 fragment revealed a total of 9 haplotypes and 13 variable sites. Out of 13 polymorphic sites, 5 were singletons. Dendrogram of phylogenetic showing genetic similarity between the two populations, but probably diversity within populations indicate the possibility to improve genetic changes and increase the resistance to environmental stresses using selection. The results of genetic distance and polymorphic site of GPX-1 in different species approved phylogenetic tree findings. Study of positive- selection process showed that selection and evolution are playing major roles in understanding the biological function of this gene.ش
Mohadeseh Namvar; Gholam Reza Dashab; Mohammad Rokouei; Hadi Faraji Arogh; Davoud Ali Saghi
Volume 19, Issue 2 , August 2017, , Pages 281-298
Abstract
This study was performed to compare different models for estimation of direct and maternal heritability and also for obtaining of genetic trend for body weight traits in Kurdi sheep of North Khorasan. The records of body weight in birth (BW, 7345 records), 3-month (3W, 5905 records), 6-month (6W ,5294 ...
Read More
This study was performed to compare different models for estimation of direct and maternal heritability and also for obtaining of genetic trend for body weight traits in Kurdi sheep of North Khorasan. The records of body weight in birth (BW, 7345 records), 3-month (3W, 5905 records), 6-month (6W ,5294 records), 9-month (9W, 3800 records) and 12-month (12W, 3863 records) weight that collected during 1990 to 2013 by Sheep Breeding Station of Shirvan were used. Estimation of genetic parameters for different traits by using of six animal models was carried out by ThrGibbsf90 software. The most suitable model for each trait was determined based on Deviance Information Criterion (DIC). The estimates of direct heritability for BW, 3W, 6W, 9W and 12W traits were 0.172± 0.0007, .0.257± 0.0007, 0.351± 0.0006, 0.120± 0.0007 and 0.131± 0.0009, respectively. In this study, the material genetic effect was significant for BW, 3W and 6W traits (P<0.01) and proportion of maternal permanent environmental variance to phenotypic variance was varied from 0.055 (12W) to 0.186 (BW). Although estimated heritability of maternal effects for body weight was lower in older ages, the result of this study showed that including maternal effects (maternal genetic and permanent environmental effect) in the statistical model could lead to more accurate estimation of genetic parameters for growth traits in all ages. The phenotypic trend for BW, 3W, 6W, 9W and 12W were 8.4, 74.6, 8.3, 54.3, 78.3 gr/year, respectively and direct additive genetic trend for mentioned traits were -0.07, 14.2, -21.9, 13.7, 24.9 gr/year, respectively. Therefore, the results of bayesian analysis are more reliable than convientioal statistical methods.
Fatemeh Mir; Mohammad Rokouei; Gholamreza Dashab; Hadi Faraji- Arough
Volume 17, Issue 2 , October 2015, , Pages 161-170
Abstract
The aim of this study was to design the base breeding plan in Sistani cattle by using of stochastic simulation. For this purpose, three population size (500, 1000, 2000 heads), three levels of herd number (5, 10, 20), two levels of selection intensity (high and low) and two levels of mating method (random ...
Read More
The aim of this study was to design the base breeding plan in Sistani cattle by using of stochastic simulation. For this purpose, three population size (500, 1000, 2000 heads), three levels of herd number (5, 10, 20), two levels of selection intensity (high and low) and two levels of mating method (random and Minimum co-ancestry) were combined together and in total 36 scenarios were compared for 30 years. Results showed that the effect of all factors on the annual genetic gain for total merit, the annual genetic gain of birth weight (except in mating method and selection intensity) and yearling weight, the annual inbreeding rate (except in herd number), generation interval (except in mating method and selection intensity) and selection accuracy of sire and dames was significant. The annual genetic gain in high selection intensity, population size of 2000 and less number of herd (5 herds) was 8.5, 93.2 and 38 percent higher than low selection intensity, population size of 500 and more number of herd (20 herds). Despite having less total genetic gain (11 percent) in Minimum co-ancestry mating, the annual inbreeding rate in this mating method was 18 percent lower than random mating. The results of this study showed that less number of herd, large population size and high selection intensity considered in breeding plans of Sistani cattle and minimum co-ancestry mating used for control of population inbreeding.
Samane Abuli; Gholam Reza Dashab; Mohammad Rokouei; Mehdi Vafaei valeh
Volume 17, Issue 1 , April 2015, , Pages 107-117
Abstract
The polymorphism in exon three of FABP4 gene and its association with growth traits of 45 Sistani (n=30) and Dashtiari (n=15) cattle were investigated. DNA extraction from the whole blood was performed and its quality was determined by electrophoresis of one percent agarose gel. Animal genotypes were ...
Read More
The polymorphism in exon three of FABP4 gene and its association with growth traits of 45 Sistani (n=30) and Dashtiari (n=15) cattle were investigated. DNA extraction from the whole blood was performed and its quality was determined by electrophoresis of one percent agarose gel. Animal genotypes were determined based on polymerase chain reaction (PCR) products and their band size electrophoresed on agarose 2.8 percent resulted from enzyme digestion by NlaIII. The pattern of bands showed three genotypes including AA, AB and BB in two Sistani and Dashtiari breeds with frequency of 67, 30 and three percent and 73, 27 and zero percent, respectively. The frequency of A and B alleles in exon three of FABP4 in Sistani and Dashtiari breeds were 82 and 18 percent and 86.5 and 13.5 percent, respectively. Heterozygosity indices including Shannon index (I), Nei’s index, observed and expected heterozygosity in Sistani and Dashtiari population were 48, 30, 30 and 30 percent and 39, 11, 27 and 24 percent, respectively. The association between genotypes and growth-related traits were significant for body weights in six, nine and 12 months of age. Therefore, this locus can be considered as a candidate gene in breeding programs for describing the variation of growth traits after weaning age in calves.